Faculty & Staff at TUC

David T. Madden

David T. Madden

College: COP

Department: Biological & Pharmaceutical Sciences

Title: Associate Professor

Phone: (707) 638-5248

Fax: (707) 638-5266

E-Mail: david.madden@tu.edu

Office: Administration & Faculty 2, Rm. 210

Institution Degree Field of Study Obtained
University of Oregon B.A. Biology, Chemistry Minor 1997
University of California at Berkeley Ph.D. Molecular and Cell Biology 2002

Fall Semester 
PHRM 601: Biological Sciences (Participating Faculty Member) 
PHRM 602: Pharmaceutical Sciences (Participating Faculty Member) 
PHRM 609: Biological Sciences (Participating Faculty Member) 
PHRM 610: Pharmaceutical Sciences (Participating Faculty Member) 

Spring Semester 
PHRM 606: Pharmaceutical Sciences (Participating Faculty Member) 
PHRM 614: Pharmaceutical Sciences (Participating Faculty Member)

The therapeutic potential of human embryonic stem cells (hESCs) is extraordinary. Without a doubt, regenerative medicines will save thousands of lives in the years to come. Before that day arrives, much needs to be learned from the cells themselves. Ultimately, if therapies based on stem cells are to be realized, these cells will have to be grown in massive quantities, with an unprecedented level of quality control to ensure that only one cell type can be found in the lot. Furthermore, the fate of hESCs is crucial to their use in new therapies. In other words, these cells must be kept alive and functional to have benefit to human patients. 

We are interested in identifying signaling pathways in hESCs that relay suboptimal growth conditions or environments to a sophisticated intracellular machinery that, when activated, causes the cell to undergo a sort of cellular suicide - or apoptosis. Identifying the triggers of cell death in hESCs would have two implications: (1) changes in the environment that would alleviate apoptosis could be obviated and (2) pharmacological interventions that prevent apoptosis could be identified. Our current approach has been to use molecular biology tools to determine the activity of a family of genes that regulate the apoptotic machinery: The so-called BCL-2 family. The activity of BCL-2 family members would point to the triggers of apoptosis in the environment, which could aid our search for optimal growth conditions for hESCs in culture, as well as hESC-derived cell types used for transplantation.

 

Madden DT, Davila-Kruger D, Melov S, Bredesen DE (2011) Human embryonic stem cells express elevated levels of multiple pro-apoptotic BCL-2 family members. PLoS One 6 e28530

Zang J, Rao RV, Spilman P, Mangada J, Xie L, Vitelli C, Gorostiza OF, Madden DT, Zeng X, Hart MJ, Bredesen DE, Galvan V (2011) Endogenously EGFP-labeled mouse embryonic stem cells. Aging and Disease 2 18 

Poksay KS, Madden DT, Peter AK, Niazi K, Banwait S, Crippen D, Bredesen DE, Rao RV (2011) Valosin-containing protein gene mutations: cellular phenotypes relevant to neurodegeneration. Journal of Molecular Neuroscience 44 91 

Birket, MJ, Orr AL, Gerencser AA, Madden DT, Viteli C, Swistowski A, Brand MD, Zeng X (2011) A reduction in ATP demand and mitochondrial activity with neural differentiation of human embryonic stem cells. Journal of Cell Science124 348 

Madden, DT, Egger, L, and Bredesen, DE (2007) A calpain-like protease inhbits autophagic cell death. Autophagy 3519 

Egger, L, Madden, DT, Rheme, C, Rao, RV, and Bredesen, DE (2007) Endoplasmic reticulum stress-induced cell death mediated by the proteasome. Cell Death and Differentiation 14 1172 

Sun, Y, Kaksonen, M, Madden, DT, Schekman, R, and Drubin, DG (2005) Interaction of Sla2p's ANTH domain with PtdIns(4,5)P2 is important for actin-dependent endocytic internalization. Molecular Biology of the Cell 16 717 

Supek, F*, Madden, DT*, Hamamoto, S, Orci, L, and Schekman, R (2002) Sec16p potentiates the action of COPII proteins to bud transport vesicles. Journal of Cell Biology 158 1029 
(*Note: these authors contributed equally to the work) 

Antonny, B, Madden, D, Hamamoto, S, Orci, L, and Schekman, R (2001) Dynamics of the COPII coat with GTP and stable analogues. Nature Cell Biology 3 531 

Lowther, WT, Orville, AM, Madden, DT, Lim, S, Rich, DH, and Matthews, BW (1999) Structural insights into the reaction mechanism of dinuclear-Co(II) methionine aminopeptidase. Journal of Inorganic Biochemistry 74 255 

Lowther, WT, Orville, AM, Madden, DT, Lim, S, Rich, DH, and Matthews, BW (1999) Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis. Biochemistry 38 7678 

Adjunct Assistant Professor, Buck Institute for Research on Aging, Novato CA

1994 
Robert C. Terwilliger Scholarship, Oregon Institute of Marine Biology 

1996 
Phi Beta Kappa, Alpha of Oregon Chapter, University of Oregon 

1997 
Honors in Biology, University of Oregon 

1997 
Robert D. Clark Honors College Graduate, University of Oregon 

2003-2004
 
Competitive NIH-NIA T32 Fellowship, #5 T32 AG000278-02, Cynthia Kenyon (UCSF) 

2004 
NRSA F32 Fellowship, #1 F32 NS047938-01 (declined for Hillblom Fellowshiop) 

2004-2007
 
Larry L. Hillblom Foundation Fellow 

2007-2009
 
California Institute of Regenerative Medicine SEED Grant-sponsored Fellow

2012

Rho Chi Society, Delta Delta Chapter, Touro University California

Employer Title From - To
Dr. Brian Matthews, Inst. of Mol. Biol, Univ. of Oregon Research student 1994-1997
Dr. Randy Schekman, MCB, UC Berkeley Graduate student 1997-2002
Dr. Dale Bredesen, Buck Institute for Research on Aging Postdoctoral Fellow 2003-2007
Dr. Dale Bredesen, Buck Institute for Research on Aging Senior Research Investigator 2007-2009
Touro University California / College of Pharmacy Assistant Professor 2009-present
Last Updated: 5/5/17