Faculty & Staff at TUC

Dr. Nathalie Garcia-Russell

Nathalie Garcia-Russell

College: COM

Department: Clinical Education

Title: Associate Professor and Assistant Dean

Phone: (707) 638-5917

Fax: (707) 638-5252

E-Mail: nathalie.garcia-russell@tu.edu

Office: Administration & Faculty1, Rm. 216

Institution Degree Field of Study Obtained
Université Paris XIII, France & UC San Diego, USA Ph.D. Biology 2000
San Diego State University, USA Post-Doc Microbial Genetics 2000-2004



Medical Microbiology – OMSI & OMSII
MSMHS program

Microbial Genetics: EFFECTS OF ENVIRONMENTAL STRESS ON SALMONELLA TYPHIMURIUM VIRULENCE AND EVOLUTION
Prophages are genomic DNA of bacteriophages (small bacterial viruses) that reside in the bacterial chromosome in a quiescent state. These small genomes can be reactivated and the newly formed viral particles may subsequently integrate the genome of a new bacterial host. Because these small transferable elements often carry virulence factor genes, they play a major role not only in bacterial evolution but also in the evolution of infectious diseases. In my laboratory we investigate the effects of the bacterial environment on the emergence of new virulent strains by studying (1) the induction, i.e. reactivation, of these transferable elements, and (2) the expression of prophage carried virulence genes, in response to environmental stress.

High Altitude Physiology: ACCLIMATIZATION TO HIGH ALTITUDE AND ACUTE MOUNTAIN SICKNESS (AMS)
Altitude illness is the result of an inadequate acclimatization to high altitude caused by alterations in the normal compensatory responses to low oxygen environments. The exact mechanisms accountable for Acute Mountain Sickness (AMS) symptoms are not well understood. No way has been found to predict who is likely to get sick at altitude and preventive treatments other than acetazolamide are lacking. Given that the individual differences in acclimatization to high altitude and AMS susceptibility are unclear to date, and most-likely multifactorial, we hypothesize that the physiological changes that need to occur in order to acclimatize can be facilitated by decreasing allostatic load by using OMT to optimize biomechanical structure and physiological function. The specific aim of the present project is to determine if OMM treatment, before or during high altitude exposure, can prevent and/or decrease the incidence of Acute Mountain Sickness in unacclimatized individuals sensitive to hypoxia exposure.

Publications

MICROBIAL GENETICS

Publications

- Garcia-Russell N., Elrod B., Dominguez K. Stress-induced prophage DNA replication in Salmonella enterica serovar Typhimurium. Infect Genet Evol. 2009 Sep;9(5):889-95.

- Garcia-Russell N., Orchard S.S. and Segall A.M. Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements. Methods Enzymol. 2007;421:209-26.

- Garcia-Russell N., Harmon T.G., Le T.Q., Amaladas N.H., Mathewson R.D. and Segall A.M. Unequal access of chromosomal regions to each other in Salmonella: Probing chromosome structure using phage lambda integrase-mediated long-range rearrangements. Mol. Microbiol. 2004;52(2): 329-344.

Research Presentations

- Tang M., Cabanne M., Tiglao S., Elrod B., Dominguez K., and Garcia-Russell N. Spontaneous Induction of Salmonella enterica serovar Typhimurium LT2 prophages. MEEDIG IX [Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases]. Irvine, California, USA, October 30th-November 1st 2008.

- Cabanne M., Tang M., Tiglao S., Elrod B., Dominguez K., and Garcia-Russell N. The response of the Salmonella Typhimurium prophages to environmental stress. AOA Annual Research Conference. Las Vegas, Nevada, USA, October 26th 2008. Abstract Published in January 2009: JAOA 109 (1): S03 (SOMA 08-03), 51.

- Elrod B., Dominguez K., and Garcia-Russell N. Induction of Salmonella Typhimurium prophages in response to environmental stress. Keystone Symposia Global Health Series on Molecular Evolution as a Driving Force in Infectious Diseases. Breckenridge, Colorado, USA, April 8 - 13, 2008.

- Orchard S.S., Garcia-Russell N., Ham-Zhu R. and A. M. Segall. Mutations that alter recombination at Salmonella chromosomal sites. EMBO Workshop on Cell Cycle and Cytoskeletal Elements in Bacteria – Copenhagen, Denmark, August 23-27, 2006.

- Garcia-Russell N., Charuvu S., Ham-Zhu R., Bevilacqua M., Segall A.M. SeqA limits global chromosome fluidity in Salmonella. 106th American Society for Microbiology General Meeting – Orlando, Florida, USA, May 21-25, 2006.

- Orchard S.S., Garcia-Russell N. and A. M. Segall. Influence of chromosome context on the interactions of lambda Integrase with different chromosomal locations. Gordon Research Conference on Chromosome Dynamics – New London, New Hampshire, USA, July 31- August 05, 2005 –

- Garcia-Russell N., Harmon T., Amaladas N. and Segall A.M. Probing chromosome structure in Salmonella using site-specific recombination. San Diego Microbiology Group Annual Meeting – San Diego, California, USA, May 15th 2004.

- Garcia-Russell N., Harmon T.G., Le T.Q., Amaladas N.H., Mathewson R.D. and Segall A.M. In vivo study of Salmonella chromosomal organization using lambda site specific recombination. Keystone Symposium on Bacterial Chromosomes – Santa Fe, New Mexico, USA, February 7-13, 2004.

- Garcia-Russell N., Harmon T.G., Amaladas N.H. and Segall A.M. In vivo study of chromosome organization in Salmonella Typhimurium by using the lambda recombinase system. 103rd American Society for Microbiology General Meeting – Washington, D.C., USA, May 18-22, 2003.

- Garcia-Russell N., Harmon T., Amaladas N., Mathewson R. and Segall A. Probing Salmonella chromosome superstructure using lambda site-specific recombination. Analytical Genetics Workshop – Santorini, Greece, October 1-5, 2002.

- Garcia-Russell N., Harmon T, Amaladas N., Mathewson R. and Segall A. Probing in vivo chromosome structure of Salmonella Typhimurium. Molecular Genetics of Bacteria and Phages – Cold Spring Harbor, New York, USA, August 20-25, 2002.

- Harmon T., Garcia-Russell N. and Segall A. Probing Salmonella chromosome superstructure using lambda site specific recombination. Keystone Symposium on Bacterial Chromosomes – Santa Fe, New Mexico, USA, February 7-13, 2001.


HIGH ALTITUDE PHYSIOLOGY

Publications

- Hupperets M.D., Hopkins S.R., Pronk M.G., Tiemessen I.J., Garcia N., Wagner P.D. and Powell F.L. Increased hypoxic ventilatory response during 8 weeks at 3800 m altitude. Respir.Physiol.Neurobiol. 2004;142(2-3):145-152.

- Garcia N., Hopkins S.R., Elliott A.R., Aaron E.A., Weinger M.B. and Powell F.L. Ventilatory response to 2-h sustained hypoxia in humans. Respir. Physiol. 2001;124(1):11-22.

- Powell F.L. and Garcia N. Physiological effects of intermittent hypoxia. High. Alt. Med. Biol. 2000;1(2):125-36.

- Garcia N., Hopkins S.R. and Powell F.L. Intermittent vs. continuous hypoxia: effects on ventilation and erythropoiesis in humans. Wilderness Environ. Med. 2000;11(3):172-9.

- Garcia N., Hopkins S.R. and Powell F.L. Effects of intermittent hypoxia on the isocapnic hypoxic ventilatory response and erythropoiesis in humans. Respir. Physiol. 2000;123(1-2):39-49.

- Savourey G., Garcia N., Caravel J.P., Gharib C., Pouzeratte N., Martin S. and Bittel J. Pre-adaptation, adaptation and de-adaptation to high altitude in humans: hormonal and biochemical changes at sea level. Eur. J. Appl. Physiol. Occup. Physiol. 1998;77(1-2):37-43.

- Savourey G., Guinet A., Besnard Y., Garcia N., Hanniquet A.M. and Bittel J. Are the laboratory and field conditions observations of acute mountain sickness related? Aviat. Space Environ. Med. 1997;68(10):895-9.

- Savourey G., Guinet A., Besnard Y., Garcia N., Hanniquet A.M. and Bittel J. General and local cold responses in humans after 2 weeks at high altitude. Eur. J. Appl. Physiol. Occup. Physiol. 1997;75(1):28-33.

- Savourey G., Garcia N., Besnard Y., Guinet A., Hanniquet A.M. and Bittel J. Pre-adaptation, adaptation and de-adaptation to high altitude in humans: cardio-ventilatory and hematological changes. Eur. J. Appl. Physiol. Occup. Physiol. 1996;73(6):529-35.

- Savourey G., Guinet A., Besnard Y., Garcia N., Hanniquet A.M. and Bittel J. Evaluation of the Lake Louise acute mountain sickness scoring system in a hypobaric chamber. Aviat.SpaceEnviron. Med. 1995;66(10):963-7.

- Savourey G., Garcia N., Besnard Y., Hanniquet A.M., Fine M.O. and Bittel J. Physiological changes induced by pre-adaptation to high altitude. Eur. J. Appl. Physiol. Occup. Physiol. 1994;69(3):221-7.

Research Presentations

- Abo B., Hakopian D., Morin R., Hiserote M., Wong T., Burns J., Ekholm R., Mokari B., and Garcia-Russell N. Using Osteopathic Manipulations to Decrease the Incidence of Acute Mountain Sickness. AOA Annual Research Conference. New Orleans, Louisiana, USA, November 1st 2009.

- Hupperets M.D.W., Hopkins S.R., Pronk M.G., Tiemessen I.J.H., Garcia N., Wagner P.D. and Powell F.L. Ventilatory acclimatization in humans during 8 weeks at 3,800m altitude.Experimental Biology – New Orleans, Louisiana, USA, April 20-24, 2002. FASEB J. 16(4), A66.

- Garcia N., Hopkins S.R. and Powell F.L. Effects of intermittent versus continuous hypoxia on the isocapnic hypoxic ventilatory response in man. ALA/ATS International Conference – San Diego, California, USA, April 23-28, 1999.

- Powell F.L., Garcia N. and Hopkins S.R. Ventilatory acclimatization to intermittent hypoxia in humans. International Hypoxia Symposium – Jasper Park Lodge, Alberta, Canada, February 27 to March 3, 1999.

- Garcia N., Hopkins S.R. and Powell F.L. Intermittent hypoxia as a way to induce ventilatory and hematological acclimatization to high altitude. Experimental Biology – San Francisco, California, USA, April 18-22, 1998, FASEB J. 12(5), A637.

Employer Title From - To
Touro University California Assistant Dean - Clinical Education 2013-present
Touro University California Associate Professor 2010-2013
Touro University California Assistant Professor 2005-2010
Ambry Genetics Scientist, R&D department 2004-2005
Last Updated: 5/5/17