Faculty & Staff at TUC

David J Evans

David J Evans

College: COP

Department: Biological & Pharmaceutical Sciences

Title: Professor

Phone: (707) 638-5240

Fax: (707) 638-5255

E-Mail: david.evans@tu.edu

Office: Administration & Faculty 2, Rm. 219

Institution Degree Field of Study Obtained
Manchester University, UK BSc Pharmacy 1985
Manchester University, UK PhD Pharmaceutical Microbiology 1991

Fall Semester

PHRM 601: Biological Sciences
PHRM 609: Biological Sciences

Spring Semester

PHRM 605: Biological Sciences
PHRM 613: Biological Sciences

My research concentrates on the pathogenesis of bacterial ocular infections, namely infectious keratitis (corneal infection) that is commonly associated with contact lens-wear. The Gram-negative bacterium Pseudomonas aeruginosa is a common cause of bacterial keratitis and infections involving this pathogen are often sight-threatening. My research is conducted in collaboration with Professor Suzanne Fleiszig, School of Optometry, University of California at Berkeley. Since 1995 we have jointly investigated the bacterial and host factors that participate in P. aeruginosa corneal infection. Areas of investigation include: the role of bacterial adherence, invasion, intracellular survival, and cytotoxicity towards corneal epithelial cells in pathogenesis of disease, and in particular the role of the P. aeruginosa Type-III secretion system and its effectors in these processes. Other research projects are focused on the host innate immune responses towards bacteria at initiation of infection, e.g. the role of the tear film and corneal epithelial antimicrobial and immunomodulatory defenses in normally protecting the eye from infection and excessive inflammation. Our goal is to understand the pathogenesis of infection and develop or enhance therapeutic interventions. This research is funded by grants from the National Eye Institute and National Institute for Allergy and Infectious Diseases, and by the Bill and Melinda Gates Foundation. Other support is provided by the Contact Lens and Ocular Pharmaceutical Industries.

Current Support

NIH, R01-EY011221. “Role of Corneal Epithelium in Health and Disease"
Co-Investigator, with Dr. Suzanne Fleiszig, UC Berkeley. Sept, 2010 - Aug 2014.

NIH, R01-AI079192. "Intracellular Lifestyle of Pseudomonas aeruginosa"
Co-Investigator with Dr. Suzanne Fleiszig, UC Berkeley. Dec, 2008 – Nov, 2013.

Previous Support

Bill & Melinda Gates Foundation, Seattle, WA. "The Eye as a source of 
novel broad-spectrum anti-infectives." Phase I, Grand Challenges Initiative. 
Co-Principal Investigator with Dr. Suzanne Fleiszig, UC Berkeley. Oct, 2008 – Sept, 2009.

Heimer SR, Evans DJ, Stern ME, Barbieri JT, Yahr T, Fleiszig SMJ. Pseudomonas aeruginosa utilizes the type III secreted toxin ExoS to avoid acidified compartments within epithelial cells. PLoS ONE. 2013: 8; e73111.

Heimer SR, Evans DJ, Mun JJ, Stern ME, Flesizig SMJ. Surfactant protein D contributes to ocular defense against Pseudomonas aeruginosa colonization in a murine model of dry eye disease. PLoS ONE. 2013: 8; e65797.

Evans DJ and Fleiszig SMJ. Perspective. Why does the healthy cornea resist Pseudomonas aeruginosa infection? American Journal of Ophthalmology. 2013: 155; 961-970.

Mun JJ, Tam C, Chan G, Kim, JH, Evans DJ, Fleiszig SMJ. MicroRNA-762 is upregulated in human corneal epithelial cells in response to tear Fluid and Pseudomonas aeruginosa antigens and negatively regulates the expression of host defense genes encoding RNase7 and ST2. PLoS ONE. 2013: 8; e57850.

Evans DJ and Fleiszig SMJ. Microbial keratitis: could contact lens material affect disease pathogenesis? Eye and Contact Lens. 2013: 39; 72-7. 

Tam C, Mun JJ, Evans DJ, Fleiszig SMJ. Cytokeratins mediate epithelial innate defense through their antimicrobial properties. Journal of Clinical Investigation. 2012: 122; 3665-3677.

Hritonenko V, Evans DJ, Fleiszig SMJ. Translocon-independent intracellular replication by Pseudomonas aeruginosa requires the ADP-ribosylation domain of ExoS. Microbes and Infection. 2012: 14; 1366-73.

Ramirez J, Fleiszig SMJ, Sullivan AB, Tam C, Borazjani R, Evans DJ. Traversal of Multilayered Corneal Epithelia by Cytotoxic Pseudomonas aeruginosa Requires the Phospholipase Domain of ExoU. Investigative Ophthalmology and Visual Science. 2012: 53; 448-53. Dec 13, 2011 [E pub].

Tam C, LeDue J, Mun JJ, Herzmark P, Robey EA, Evans DJ, Fleiszig SMJ. 3D quantitative imaging of unprocessed live tissue reveals epithelial defense against bacterial adhesion and subsequent traversal requires MyD88. PLoS ONE. 2011: 6; e24008. Aug 25 [E pub]. 

Hritonenko V, Mun J, Tam C, Simon NC, Barbieri JT, Evans DJ, Fleiszig SMJ. Adenylate cyclase activity of Pseudomonas aeruginosa ExoY can mediate bleb-niche formation in epithelial cells and contributes to virulence. Microbial Pathogenesis. 2011: 51; 305-12. Aug 9 [E pub].

Mun JJ, Tam C, Evans DJ, Fleiszig SMJ. Modulation of epithelial immunity by mucosal fluid. Nature Scientific Reports, 2011: 1; 8. Jun 14 [E pub].

Tran VB, Sung YS, Fleiszig SMJ, Evans DJ, Radke CJ. Dynamics of Pseudomonas aeruginosa association with anionic hydrogel surfaces in the presence of aqueous divalent-cation salts. Journal of Colloid and Interface Science, 2011: 362; 58-66. Jun 13 [E pub]. 

Tran VB, Fleiszig SMJ, Evans DJ, Radke CJ. Dynamics of Flagellar and Pilus-mediated Pseudomonas aeruginosa Surface Association with Contact Lenses. Applied and Environmental Microbiology, 2011: 77; 3644-52. Apr 15 [E pub].

Augustin DK, Heimer SR, Tam C, Li W, LeDue J, Evans DJ, Fleiszig SMJ. Role of Defensins in Corneal Epithelial Barrier Function against P. aeruginosa Traversal. Infection and Immunity, 2011: 79; 595-605. Nov 29, 2010 [E pub].

Alarcon I, Tam C, Mun J, LeDue J, Evans DJ, Fleiszig SMJ. Factors Impacting Corneal Epithelial Barrier Function Against Pseudomonas aeruginosa Traversal. Investigative Ophthalmology and Visual Science, 2011: 52; 1368-77. Nov 4, 2010 [E pub].

Angus AA, Evans DJ, Barbieri JT, Fleiszig SMJ. The ADP-Ribosylation Domain of Pseudomonas aeruginosa ExoS is Required for Membrane Bleb-Niche Formation and Bacterial Survival within Epithelial Cells. Infection and Immunity, 2010: 78; 5400-10. Aug 23 [E pub].

Fleiszig SMJ, Evans DJ. Pathogenesis of Contact Lens-Associated Microbial Keratitis. Optometry and Vision Science, 2010: 87; 225-32. E pub Feb 25. [Also Response to Letter to Editor, 2010. Fleiszig SMJ, Evans, DJ. Optometry & Vision Science, 2010: 87; 613-14].

Tam C, Mun JJ, Evans DJ, Fleiszig SMJ. The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis. Investigative Ophthalmology and Visual Science, 2010: 51; 3100-6. Feb 3 [E pub].

Mészáros K, Barnett MJ, McDonald K, Wehring H, Evans DJ, Sasaki-Hill D, Goldsmith PC, Knapp KK. Progress Examination for Assessing Students’ Readiness for Advanced Pharmacy Practice Experiences. American Journal of Pharmaceutical Education, 2009: 73; Article 109. 

Alarcon I, Kwan L, Yu C, Evans DJ, Fleiszig SMJ. The role of corneal epithelial basement membrane in ocular defense against Pseudomonas aeruginosa. Infection and Immunity, 2009: 77; 3264-71. June 8 [E pub]. 

Mun JJ, Tam C, Kowbel D, Hawgood S, Barnett MJ, Evans DJ, Fleiszig SMJ. Clearance of Pseudomonas aeruginosa from a Healthy Ocular Surface Involves Surfactant Protein-D and is Compromised by Bacterial Elastase in a Murine “Null-Infection” Model. Infection and Immunity, 2009: 77; 2392-2398. April 6 [E pub].

Alarcon I, Evans DJ, Fleiszig SMJ. The Role of Twitching Motility in Pseudomonas aeruginosa Exit From and Translocation of Corneal Epithelial Cells. Investigative Ophthalmology and Visual Science, 2009: 50; 2237-2244. Jan 10 [E pub]. 

Thompson DF, Farmer KC, Beall DG, Evans DJ, Melchert RB, Ross LA, Schmoll BJ. Identifying perceptions of professionalism in pharmacy using a four-frame leadership model. American Journal of Pharmaceutical Education, 2008: 72; Article 90, 1-8. 

Ni M, Tam C, Verma A, Ramphal R, Hawgood S, Evans DJ, Fleiszig SMJ. Expression of Surfactant Protein D in Human Corneal Epithelial Cells is Upregulated by Pseudomonas aeruginosa. FEMS Immunology and Medical Microbiology, 2008: 54; 177-184.

Angus AA, Ackerman-Lee A, Augustin DK, Lee EJ, Evans DJ, Fleiszig SMJ. Pseudomonas aeruginosa Induces Membrane Blebs in Epithelial Cells Which are Utilized as a Niche for Intracellular Replication and Motility. Infection and Immunity, 2008: 76; 1992-2001.

Tam C, Lewis S, Li W, Lee E, Evans DJ, Fleiszig SMJ. Mutation of the Phospholipase Catalytic Domain of the Pseudomonas aeruginosa Cytotoxin ExoU Abolishes Colonization Promoting Activity and Reduces Corneal Disease Severity. Experimental Eye Research, 2007: 85; 799-805.

Evans DJ, McNamara NA, Fleiszig SMJ. Bacterial keratitis: Dissecting host-pathogen interactions at the ocular surface. Ocular Surface Journal, 2007: 5; 213-27.

Maltseva I, Fleiszig SMJ, Evans DJ, Kerr, S, Sidhu S, McNamara NA, Basbaum C. Exposure of Human Corneal Epithelial Cells to Contact Lenses In Vitro Suppresses the Upregulation of Human Beta-Defensin-2 in Response to Antigens of Pseudomonas aeruginosa. Experimental Eye Research, 2007: 85; 142-153.

Kwong MSF, Evans DJ, Ni M, Cowell BA, Fleiszig SMJ. Human Tear Fluid Protects Against Pseudomonas aeruginosa Keratitis in a Murine Experimental Model. Infection and Immunity, 2007: 75; 2325-32.

Zolfaghar I, Evans DJ, Ronaghi R, Fleiszig SMJ. Type III-Secretion-Dependent Modulation of Innate Immunity as one of Multiple Factors Regulated by Pseudomonas aeruginosa RetS. Infection and Immunity, 2006: 74; 3880-9.

Zolfaghar I, Angus AA, Kang PJ, To A, Evans DJ, Fleiszig SMJ. Mutation of retS, encoding a putative hybrid two-component regulatory protein in Pseudomonas aeruginosa, attenuates multiple virulence mechanisms. Microbes and Infection, 2005: 7; 1305-16.

Cowell BA, Evans DJ, Fleiszig SMJ. Actin cytoskeleton disruption by ExoY and its effects on Pseudomonas aeruginosa invasion. FEMS Microbiology Letters. 2005: 250; 71-6.

Ni M, Evans DJ, Hawgood S, Anders EM, Sack RA, Fleiszig SMJ. Surfactant protein D is present in human tear fluid and the cornea and inhibits epithelial cell invasion by Pseudomonas aeruginosa. Infection and Immunity, 2005: 73; 2147-56.

Lee EJ, Evans DJ, Fleiszig SMJ. Role of Pseudomonas aeruginosa ExsA in penetration through corneal epithelium in a novel in vivo model. Investigative Ophthalmology and Visual Science, 2003: 44; 5220-27.

Zolfaghar I, Evans DJ, Fleiszig SMJ. Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa. Infection and Immunity, 2003: 71; 5389-93.

Lee EJ, Cowell BA, Evans DJ, Fleiszig SMJ. Contribution of ExsA-regulated factors towards corneal infection by cytotoxic and invasive Pseudomonas aeruginosa in a murine scarification model. Investigative Ophthalmology and Visual Science, 2003: 44; 3892-98.

Fleiszig SMJ, Evans DJ. Contact Lens Infections: Can they ever be eradicated? Eye and Contact Lens, 2003: 29; S67-71.

Fleiszig SMJ, Kwong M, Evans DJ. Modification of Pseudomonas aeruginosa interactions with corneal epithelial cells by human tear fluid. Infection and Immunity, 2003: 71; 3866-74.

Fleiszig SMJ, McNamara NA, Evans DJ. The Tear Film and Defense against Infection. Advances in Experimental Medicine and Biology, 2002: 506; 523-30.

Evans DJ, Kuo T, Kwong M, Van R, Fleiszig SMJ. Pseudomonas aeruginosa strains with lipopolysaccharide defects exhibit reduced intracellular viability after invasion of corneal epithelial cells. Experimental Eye Research, 2002: 75; 635-43.

Evans DJ, Kuo TC, Kwong M, Van R, Fleiszig, SMJ. Mutation of csk, encoding the C-terminal Src kinase, reduces Pseudomonas aeruginosa internalization by mammalian cells and enhances bacterial cytotoxicity. Microbial Pathogenesis, 2002: 33; 135-43.

Evans DJ, Maltseva IA, Wu J, Fleiszig SMJ. Pseudomonas aeruginosa internalization by corneal epithelial cells involves MEK and ERK signal transduction proteins. FEMS Microbiology Letters, 2002: 213; 73-9.

Fleiszig SMJ, Evans DJ. The pathogenesis of bacterial keratitis: studies with Pseudomonas aeruginosa. Clinical and Experimental Optometry, 2002: 85; 271-8.

Dean CR, Franklund CV, Retief JD, Coyne MJ Jr, Hatano K, Evans DJ, Pier GB, Goldberg JB. Characterization of the serogroup O11 O-antigen locus of Pseudomonas aeruginosa PA103. Journal of Bacteriology, 1999: 181; 4275-84.

Lee A, Chow D, Haus B, Tseng W, Evans DJ, Fleiszig S, Chandy G, Machen T. Airway epithelial tight junctions and binding and cytotoxicity of Pseudomonas aeruginosa. American Journal of Physiology [Lung Cellular and Molecular Physiology], 1999: 277; L204-17.

Evans DJ, Matsumoto PS, Widdicombe JH, Maminishkis AA, Miller SS. Pseudomonas aeruginosa induces changes in fluid transport across airway surface epithelia. American Journal of Physiology [Cell Physiology], 1998: 275; C1284-90.

Evans DJ, Frank DW, Finck-Barbançon V, Wu C, Fleiszig SMJ. Pseudomonas aeruginosa invasion and cytotoxicity are independent events, both of which involve protein tyrosine kinase activity. Infection and Immunity, 1998: 66; 1453-9.

Fleiszig SMJ, Evans DJ, Do N, Vallas V, Shin S, Mostov K. Epithelial cell polarity affects susceptibility to Pseudomonas aeruginosa invasion and cytotoxicity. Infection and Immunity, 1997: 65; 2861-7. 

Fleiszig SMJ, Evans DJ, Zaidi TS, Mowrey-McKee MF, Payor RE, Vallas V, Muller E, Pier GB. Factors affecting Staphylococcus epidermidis adhesion to contact lenses. Optometry and Vision Science, 1996: 73; 590-4.

Fleiszig SMJ, Zaidi TS, Preston MJ, Grout M, Evans DJ, Pier GB. Cytotoxicity and corneal epithelial cell invasion by isolates of Pseudomonas aeruginosa. Infection and Immunity, 1996: 64; 2288-94.

Meluleni GJ, Grout M, Evans DJ, Pier GB. Mucoid Pseudomonas aeruginosa growing in a biofilm in-vitro are killed by opsonic antibodies to the mucoid exopolysaccharide capsule but not by antibodies produced during chronic lung infection in cystic fibrosis patients. Journal of Immunology, 1995: 155; 2029-38.

Evans DJ, Pier GB, Coyne MJ Jr, Goldberg JB. The rfb locus from Pseudomonas aeruginosa strain PA103 promotes the expression of O antigen by both LPS-rough and LPS-smooth isolates from cystic fibrosis patients. Molecular Microbiology, 1994: 13; 427-34.

Gilbert P, Evans DJ, Brown MRW. Formation and dispersal of bacterial biofilms in-vivo and in-situ. Journal of Applied Bacteriology, 1993. 74: 67-78S.

Hodgson A, Evans DJ, Brown MRW, Gilbert P. Involvement of cellular division cycle in the susceptibility of Escherichia coli to cold- and osmotic shock. Microbios, 1992: 72; 175-82.

Gilbert P, Evans DJ, Evans E, Duguid IG, Brown MRW. Surface characteristics and adhesion of Escherichia coli and Staphylococcus epidermidis. Journal of Applied Bacteriology, 1991: 71;72-7.

Evans DJ, Allison DG, Brown MRW, Gilbert P. Susceptibility of Pseudomonas aeruginosa and Escherichia coli biofilms towards ciprofloxacin: effect of specific growth rate. Journal of Antimicrobial Chemotherapy, 1991: 27; 177-84.

Allison DG, Brown MRW, Evans DJ, Gilbert P. Surface hydrophobicity and dispersal of Pseudomonas aeruginosa from biofilms. FEMS Microbiology Letters, 1990: 71; 101-4.

Evans DJ, Allison DG, Brown MRW, Gilbert P. Effect of growth rate on resistance of Gram-negative biofilms to cetrimide. Journal of Antimicrobial Chemotherapy, 1990: 26; 473-8.

Evans DJ, Brown MRW, Allison DG, Gilbert P. Susceptibility of bacterial biofilms to tobramycin: role of specific growth rate and phase in the division cycle. Journal of Antimicrobial Chemotherapy, 1990: 25; 585-91.

Allison DG, Evans DJ, Brown MRW, Gilbert P. Possible involvement of the division cycle in dispersal of Escherichia coli from biofilms. Journal of Bacteriology, 1990: 172; 1667-9.

Gilbert P, Allison DG, Evans DJ, Handley PS, Brown MRW. Growth rate control of adherent bacterial populations. Applied and Environmental Microbiology, 1989: 55; 1308-11.

  • 1998 Teacher of the Year, Touro University CA College of Osteopathic Medicine
  • 1999 Teacher of the Year, Touro University CA College of Osteopathic Medicine
  • 2006 Academic Leadership Fellows Program, American Association of Colleges of Pharmacy
  • 2009 Excellence in Assessment Award. American Association of Colleges of Pharmacy
  • 2013 Professor of the Year, Biological Sciences. Touro University CA College of Pharmacy
Employer Title From - To
Harvard Medical School & Brigham and Women’s Hospital Research Fellow 1991 - 1993
Harvard Medical School & Brigham and Women’s Hospital Instructor in Medicine 1993 - 1994
School of Optometry, University of California at Berkeley Clinical Instructor 1994 - 1995
School of Optometry, University of California at Berkeley Assistant Clinical Professor 1995 - 1998
School of Optometry, University of California Berkeley Assistant Research Scientist 1995 - 2008
Touro University California, College of Osteopathic Medicine Assistant Professor 1998 - 2000
Touro University California, College of Osteopathic Medicine Associate Professor 2000 - 2005
University of Melbourne, Dept. Microbiology & Immunology Visiting Research Fellow 2001 - 2002
Touro University California, College of Pharmacy Associate Professor 2005-2007
Touro University California, College of Pharmacy Professor 2007 - present
School of Optometry, University of California Berkeley Associate Research Scientist 2008 - present
Touro University California, College of Pharmacy Department Chair, Biological Sciences 2008 - 2011
Touro University California, College of Pharmacy Program Director, Master of Science in Medical Health Sciences 2013-present 

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Last Updated: 3/15/16