Education

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Teaching

Fall Semester

TUCA-BSCI-608-FA-05 Introduction to Biochemistry and Tissues (Participating Faculty member)

PA600-05 - 08 Basic Science Foundations (Participating Faculty member)

Spring Semester

TUCA-BSCI-610-SP-06 - 08 Basic Science Foundations

Molecular Medicine (Unit Coordinator)

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Research Interests

Website: Structural Biology Lab
We are using biochemical and biophysical tools for drug discovery against the Human Immunodeficiency Virus fusion mechanism. Drugs in this class have the potential to prevent the infection of T-cells by invading virus, and are so far limited to peptides that have poor pharmaceutical and bioavailability profiles. We are using techniques such as bio-inorganic chemistry, fluorescence, NMR spectroscopy and solid state biosensors to develop methodology allowing detection of small molecules targeting the viral fusion domain. Such compounds have the potential to be developed into effective drugs.

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Honors & Awards

1974-1976 Rhodesian Government University Scholarship, Rhodes University

1975-1976 Good Hope Textile Corporation Bursary for Chemistry, Rhodes University

1975 Sydney Cruise Memorial Prize for Mathematics, Rhodes University

1976 David Williams Memorial Prize for Mathematics, Rhodes University

1980 Recipient of Award for Distinction, Weizmann Institute of Science

1984 Regent's fellowship, University of California, Berkeley

2008 Dean's Recognition for Excellence in Teaching, UCSF School of Pharmacy

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Publications

• Gochin, M. and Moss, J.R., "On the Structure of H2Os3(CO)12", Organometallic Chemistry 192, 409 (1980)

• Degani, H., Gochin, M., Karlish, S.J.D. and Schechter, Y., "EPR Studies and Insulin-like Effects of Vanadium in Rat Adipocytes", Biochemistry 20, 5795 (1981).

• Gochin, M. and Degani, H.," 1H NMR Studies of a Two-Iron Two-Sulphur Ferredoxin from Halobacterium of the Dead Sea", J. Inorg. Biochem. 25, 151 (1985).

• Gochin, M., James, T.L. and Shafer, R.H., "In Vivo 19F NMR of 5-fluorouracil Incorporation into RNA and Metabolites in E. coli cells", Biochim. Biophys. Acta 804, 118 (1984).

• Gochin. M., Weitekamp, D.P. and Pines, A., "A SHARP Method for High Resolution NMR of Heteronuclear Spin Systems in Inhomogeneous Fields", J. Magn. Reson. 63, 431 (1985).

• Gochin, M. and Pines, A., "High Resolution NMR with a Surface Coil", J. Am. Chem. Soc. 107, 7193 (1985).

• Gochin, M., Schenker, K.V., Zimmerman, H. and Pines, A., "Determination of Dipole-dipole Couplings in Oriented n-Hexane by Two-dimensional NMR", J. Am. Chem. Soc. 108, 6813 (1986.

• Gochin, M., Hugi-Cleary, D., Zimmerman, H. and Pines, A., "Two-dimensional Multiple Quantum NMR of Isotopic Mixtures in Liquid Crystals", Mol. Phys. 60, 205 (1987).

• Gochin, M., Zimmerman, H. and Pines, A., "Assignment of Dipolar Couplings and Estimation of Conformational Probabilities in Partially Oriented Hexane", Chem. Phys. Lett. 137, 51 (1987).

• James, T.L., Kerwood, D.J., Gochin, M., Mills, P.A., Borgias, B.A. and Basus, V.J., "Refinement of Three-dimensional Protein and DNA Structures in Solution from Two-dimensional NMR Spectra", 4th Cyprus Conference on New Methods in Drug Research (1989), Proceedings, Ed. A. Makriannis.

• Borgias, B.A., Gochin, M., Kerwood, D.J. and James, T.L., "Relaxation Matrix Analysis of 2D NMR Data",Prog. Nucl. Magn. Reson. Spectr. 22, 83 (1990.

• Gochin, M., Pines, A., Rosen, M.E., Rucker, S.P. and Schmidt, C., "Two-dimensional NMR Studies of Flexible Molecules in Liquid Crystals: Orientations and Conformations of n_Hexane:, Mol. Phys. 69, 671 (1990).

• Gochin, M., Zon, G. and James, T.L., "Solution Structure of [d(AC)4•d(GT)4] via Two-dimensional COSY and 2D NOE Spectroscopy", Biochemistry 22, 11161 (1990).

• Gochin, M. and James, T.L., "Restrained Molecular Dynamics Simulations on [d(AC)4•d(GT)4] using NMR Constraints Derived from Two-dimensional NOE and DQF-COSY Experiments", Biochemistry 29, 11172 (1990).

• Gochin, M. "Determination of Proton Transverse Relaxation Times in Homonuclear Coupled Spin Systems",J. Magn. Reson. 95, 299 (1991).

• Gochin. M. and Roder, H., "The use of Pseudocontact shifts in NMR Structure Determination; Application to Wild-type and mutant Cytochrome c", Protein Science, 4, 296 (1995).

• Hom, K., Gochin, M., Peumans, W.J. and Shine, N., "Ligand-Induced Perturbations in Urtica Dioica Agglutinin", FEBS Letts, 361, 157 (1995).

• Gochin, M. and Roder, H., "Use of the Pseudocontact Shift as a Structural Constraint for Macromolecules in Solution", Bulletin of Magnetic Resonance, 1995.

• Gochin, M, " Nuclear Magnetic Resonance Studies of a Paramagnetic Metallo-DNA Complex", J. Am. Chem. Soc., 119: 3377-8 (1997).

• Gochin, M, "Nuclear Magnetic Resonance Characterization of a Paramagnetic DNA-Drug complex with High Spin Cobalt: Assignment of 1H and 31P Spectra and Determination of Electronic, Spectroscopic and Molecular Properties", J. Biomolec. NMR, 12:243-257 (1998).

• Desvaux, H and Gochin, M. " Coherence Transfer Between Nuclear Spins in Paramagnetic Systems: Effects of Nucleus-Electron Dipole-Dipole Cross-Correlation", Mol. Phys., 9: 1317-33 (1999).

• Tu, K. and Gochin, M., "Structure Determination by Restrained Molecular Dynamics using NMR Pseudocontact Shifts as Experimentally Determined Constraints", J. Am. Chem. Soc., 121: 9276-85 (1999).

• Buchko, G.W., Daughdrill,G.W., de Lorimer, R., Rao, S., Isern, N.G., Lingbeck, J., Taylor, J-S., Wold, M.S., Gochin, M., Spicer, L.D., Lowry, D.F., and Kennedy, M.A.. "Interactions of Human Nucleotide Excision Repair Protein XPA with RPA70 and DNA: Chemical Shift Mapping and 15N NMR Relaxation Studies", Biochemistry, 38:15116-28 (1999).

• Gochin, M, "A High Resolution Structure of a DNA-Chromomycin-Cobalt Complex Determined from Paramagnetic Shifts in Nuclear Magnetic Resonance", Structure with Folding and Design, 8: 441-52 (2000).

• Gochin, M., Khorosheva, V. and Case, M.A., “Structural Characterization of a Paramagnetic Metal Ion – Assembled Three Stranded a-Helical Coiled Coil”, J. Am. Chem. Soc., 124, 11018-28 (2002).

• Gochin, M., Guy, R.K. and Case, M.A. “A metallopeptide assembly of the HIV-1 gp41 coiled coil is an ideal receptor in fluorescence detection of ligand binding”, Angew. Chem. Int. Ed., 42: 5325-8 (2003).

• Gochin, M., Savage, R., Hinckley, S. and Cai, L. “A fluorescence assay for rapid detection of ligand binding affinity to HIV-1 gp41”, Biol. Chem., 387: 477-83 (2006)

• Cai, L. and Gochin, M. “A Novel Fluorescence Intensity Screening Assay Identifies New Low Molecular Weight Inhibitors of the gp41 Coiled Coil Domain of HIV-1”, Antimicrob. Agents Chemotherapy, 51: 2388-95 (2007)

• Cai, L. and Gochin, M. “Colloidal aggregate detection by rapid fluorescence measurement of liquid surface curvature changes in multi-well plates”, J. Biomol. Screen. 12(7):966-71 (2007)

• Hou, Y. and Gochin, M. “An Artificial Ion Channel Biosensor in Human Immunodeficiency Virus gp41 Drug Sensing”, Anal. Chem., 80(15):5924-9 (2008)

• Balogh E, Wu D, Zhou G, Gochin, M. “NMR Second Site Screening for Structure Determination of Ligands Bound in the Hydrophobic Pocket of HIV-1 gp41.”, J Am Chem Soc. Feb 10. [Epub ahead of print] (2009)

• Cai L, Balogh E, Gochin M. “A stable extended HIV-1 gp41 coiled coil as an effective target in an assay for high affinity fusion inhibitors.” Antimicrob Agents Chemother. 2009 Apr 13. [Epub ahead of print] (2009)

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Research Grants

Current

National Institutes of Health / National Institute of Neurological Disorders and Stroke (Roadmap) NS059403-S1, 8/1/2008-2/21/2010 ($25,000 direct costs, Supplement) Application of HIV-1 fusion inhibitor assay at MLSCN, project approval April 2, 2009

National Institutes of Health / National Institute of Neurological Disorders and Stroke (Roadmap) NS059403 ($100,000 direct costs)3/1/2007-2/28-2010 "A high throughput screening assay for HIV-1 fusion inhibitors", Miriam Gochin, Principal Investigator

Previous

University-wide AIDS Research Program, State of California ID05-TOURO-041 ($100,000 direct costs) 9/1/2005-8/31/2008 “A biosensor for detection of HIV-1 fusion inhibitors”, Miriam Gochin, Principal Investigator

National Institutes of Health / National Institute of AIDS and Infectious Diseases AI060361 ($300,000 direct costs)5/1/2004 – 4/31/2006 “Metallopeptides in HIV-1 Fusion Inhibition”, Miriam Gochin, Principal Investigator

American Foundation for AIDS Research 106388-33-RGGN ($90,000 direct costs) 1/1/2003-12/31/2003 “A Mimic of the gp41 Coiled Coil in Inhibition of HIV”, Miriam Gochin, Principal Investigator

Pacific Dental Research Foundation PDRF522 ($3604) 7/1/2003-7/1/2004 “Expression of HIV-1 gp41 Peptides in E. coli” Pacific Dental Research Foundation, Miriam Gochin, Principal Investigator

University of the Pacific School of Dentistry DRES-023 ($3000)2002 “NMR Studies of Designed Metal-binding Peptides”, Miriam Gochin, Principal Investigator

University-wide AIDS Research Program, State of California R00-UP-092 ($150,000 direct costs) 7/1/2000 – 6/31/2002 “Targeting HIV by Disruption of the Fusion Mechanism”, Miriam Gochin, Principal Investigator

National Institutes of Health / National Institute of General Medical Sciences GM53164 ($375,000 direct costs) 7/1/1995 - 6/30/1999 “Paramagnetic Ions in NMR Structure Determination”, Miriam Gochin, Principal Investigator

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